AmpFLSTR™ Yfiler™ PCR Amplification Kit

Catalog number:  4359513

Applied Biosystems™  Related applications: Forensics

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The AmpFLSTR® Yfiler® PCR Amplification Kit permits the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive results with autosomal STRs.

Key Features of the AmpFLSTR® Yfiler® PCR Amplification Kit:

• Analyze trace quantities of male DNA that may be present in a large background of female DNA
• Combine the inherent male specificity of Y-STRs with the robust performance of an AmpFLSTR® kit
• Amplify 17 Y-STR loci in a single PCR reaction which yields the highest discriminatory capacity of any commercially available Y-STR kit
• Search the user-friendly on-line haplotype database to easily determine the frequency of any Yfiler® kit haplotype
• Use this powerful tool for the analysis of cases that contain a suspect(s)

Combines the Inherent Male Specificity of Y-STRs with the Robust Performance of an AmpFLSTR® Kit
Mixtures of body fluids and/or tissues from a male and female are frequently encountered with sexual assault casework and other violent crimes. These mixed samples often contain trace quantities of male DNA present in a large background of female DNA. Typical samples can include vaginal swabs containing a small number of sperm cells, body swabs from female victims of oral assaults, and fingernail scrapings/clippings from female victims of violent assaults. Standard autosomal STR analysis may be unable to detect the minor male contributor in these types of samples, while the Yfiler® kit can routinely detect a minor male contributor present at less than 1:1000.

Provides the Highest Discriminatory Capacity of Any Commercially Available Y-STR Kit
The AmpFLSTR® Yfiler® PCR Amplification Kit amplifies 17 Y-STR loci in a single PCR reaction using 5-dye chemistry to provide the highest discriminatory capacity of any commercially available Y-STR kit. The Yfiler® kit can distinguish more than 98% of all unrelated males from one another, and therefore offers the discriminatory power necessary to conduct meaningful forensic DNA analysis.

A Powerful Tool for Suspect Casework
The Yfiler® kit is a powerful tool for the analysis of cases that contain a suspect(s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler® kit is a powerful tool that can be used to include or exclude a suspect(s) associated with an investigation.

Complete Validated Solution
Applied Biosystems® offers a complete solution for your Human Identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® kits, instrumentation, software, and consumables. The Yfiler® kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

Note: For Forensic or Paternity Use Only.

Important Notice
For In Vitro Diagnostic Use.


Amplicon Size: <340 bp
For Use With (Equipment): 310 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130 Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, GeneAmp® 9600, GeneAmp™ 9700
Label or Dye: FAM™, LIZ™, NED™, PET™, VIC®
Matrix and Size Standards: GeneScan™ 500 LIZ® Size Standard, Matrix Standard Set DS-33
Polymerase: AmpliTaq Gold® DNA Polymerase
Product Line: AmpFLSTR™, Yfiler®
Product Size: 100 reactions
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
Sample Volume: 25 µL⁄reaction
Technique: STR (Short Tandem Repeat) Analysis
Shipping Condition: Dry Ice

Contents & storage

• AmpFLSTR® PCR Reaction Mix
• AmpFLSTR® Yfiler® Primer Set
• AmpFLSTR® Control DNA 007
• AmpFLSTR® Control DNA 9947A
Store at 2 to 8°C.

• AmpliTaq Gold® DNA Polymerase
Store at -15 to -25°C

• AmpFLSTR® Yfiler® Allelic Ladder
Store at -15 to -25°C upon receipt, 2 to 8°C after initial use


Manuals & protocols