TaqMan® Fast Universal PCR Master Mix (2X), no AmpErase® UNG

Catalog number: 4366072

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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TaqMan® Fast Universal Master Mix (2x), No AmpErase® UNG delivers results in 40 minutes for 40 cycles of PCR in a 20 µL reaction volume using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems. The optimized formulation contains AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX™ dye serves as a passive internal reference to normalize non-PCR–related fluorescence fluctuations, for superb precision on Applied Biosystems® real-time PCR instruments.

Convenience: Everything you need for TaqMan®-based qPCR amplification and detection in a single-tube format. TaqMan® Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility: TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG is compatible with TaqMan® Gene Expression assays.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System, StepOnePlus™, Fast Mode, StepOne™, Fast Mode, 7900HT Fast System
Reaction Speed: Fast
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: AmpliTaq® DNA Polymerase
Product Size: 500 reactions
Passive Reference Dye: ROX (Pre-mixed)
Sample Type (Specific): cDNA, DNA (Genomic)
Primer-Probe Compatibility: TaqMan®

Contents & storage

TaqMan® Fast Universal PCR Master Mix, No AmpErase® UNG Kit contains 2 packs of 2 tubes of 1.25 mL each sufficient for 500 20 µL PCR reactions. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).


Manuals & protocols

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