Silencer® Select GAPDH Positive Control siRNA

Catalog number: 4390850

Ambion™  Related applications: RNAi | Transfection

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Description

Ambion® Silencer® SelectGAPDH Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided.

• Premium-quality siRNA, highly purified and ready to use
• Functionally tested in several common cell lines
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer® Select GAPDH Positive Control siRNA:
The Silencer® SelectGAPDH positive control is extensively validated and an ideal "test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion® KDalert™ GAPDH Assay Kit.

Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.

Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

RNAi Type: siRNA
Target Gene: GAPDH
Control Type: Gene Knockdown Positive Control
Label or Dye: Unconjugated
Product Size: 40 nmol
Shipping Condition: Room Temperature

Contents & storage

The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.

Documents

Manuals & protocols

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