MeltDoctor™ HRM Reagent Kit

Catalog number: 4425557

Applied Biosystems™  Related applications: Genotyping & Genomic Profiling | Methylation Analysis | Real Time PCR (qPCR)

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Description

Applied Biosystems® MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
This kit includes:
• AmpliTaq Gold® 360 DNA Polymerase which, when combined with AmpliTaq Gold® 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp® dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO® 9 double-stranded nucleic acid stain developed by Molecular Probes
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Mutation Scanning, HRM (High Resolution Melting) Analysis
Polymerase: AmpliTaq Gold® 360 DNA Polymerase
Label or Dye: SYTO® 9, HRM (High Resolution Melting) Dye
Product Size: 1 kit
Detection Method: Fluorescent
Genotyping Target: Epigenetic Targets, SNPs (Unknown or Numerous)
Sample Type (General): DNA
Sample Type (Specific): cDNA, DNA (Genomic)
For Use With (Equipment): 7500 Fast System, 7900HT Fast System

Contents & storage

This kit includes: AmpliTaq Gold® 360 DNA Polymerase, AmpliTaq Gold® 360 Buffer, 360 GC Enhancer, GeneAmp® dNTP Blend, MeltDoctor™ HRM Dye (20x)
Store at -15° to -25°

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Documents

Manuals & protocols