The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan® primer⁄probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold® DNA Polymerase, and a buffer mix.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Primer-probe
Form
Liquid
Format
Tube
PCR Method
1-step RT-qPCR, Multiplex qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Speed
Standard
For Use With (Equipment)
7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Passive Reference Dye
ROX (Pre-mixed)
Product Line
Path-ID™
Product Type
One-Step RT-PCR Kit
Reverse Transcriptase
ArrayScript™
Sufficient For
100 Reactions
For Use With (Application)
Pathogen Detection, Virus Detection
Hot Start
Built-In Hot Start
No. of Reactions
100 Reactions
Sample Type
Total RNA, Viral RNA, mRNA
Contents & Storage
• 1.375 mL of 2X Multiplex RT-PCR Buffer • 280 μL of 10X Multiplex Enzyme Mix including AmpliTaq Gold and ArrayScript • 1.75 mL of Nuclease-free water • Store the kit at -20°C; the Nuclease-free water may be stored at -20°C, 4°C, or room temperature
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).