SOLiD™ ChIP-Seq Kit, with ChIP magnet

Catalog number: 4449638

Applied Biosystems™  Related applications: Chromatin Biology

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Description

The SOLiD™ ChIP Seq Kit is a complete and optimized system for genome wide Chromatin Immunoprecipitation (ChIP) analysis on SOLiD™ and includes ChIP preparation reagents and library generation reagents for 20 samples along with a protocol optimized for library preparation with reduced amount of DNA from 1-10ng samples. Chromatin immunoprecipitation (ChIP) experiments examine histone modifications and genomic DNA sequences bound to specific regulatory proteins and when combined with next generation sequencing is a powerful method to examine genome wide protein DNA interactions. This configuration also includes the DynaMAG®-PCR magnet for processing ChIP samples.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Chromatin Immunoprecipitation
Product Size: 1 kit
Number of Reactions: 20 Preps
For Use With (Equipment): SOLiD™

Contents & storage

The kit contains 1.25M Glycine ,Lysis Buffe, Dilution Buffer, IP Buffer 1, IP Buffer 2, Dynabeads® Protein A⁄G Mix, Mouse IgG, Rabbit IgG, 20 mg⁄ml Proteinase K, 200X Protease Inhibitors, Reverse Crosslinking Buffer, DNA Purification Buffer, DNA Purification Magnetic Beads, DNA Wash Buffer and DNA Elution Buffer. Store Glycine, IP Buffer 1, IP Buffer 2, Dynabeads® Protein A⁄G Mix, Reverse Crosslinking Buffer, DNA Purification Buffer, DNA Purification Magnetic Beads, DNA Wash Buffer and DNA Elution Buffer at 4°C. Store Lysis Buffer, Dilution Buffer, Mouse IgG, Rabbit IgG, Proteinase K, and Protease Inhibitors at -20°C. Guranteed for 6 months when properly stored.
This kit also includes the DynaMAG®-PCR magnet. Store magnet at room temperature.

Documents

Manuals & protocols