TaqMan® OpenArray® Digital PCR Kit

Catalog number: 4458070

Applied Biosystems™  Related applications: Digital PCR | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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The TaqMan® OpenArray® Digital PCR Kit enables the accurate and sensitive quantitation of materials beyond the limits of conventional PCR. The kit includes TaqMan® OpenArray® Digital PCR Plates and 2X TaqMan® OpenArray® Digital Master Mix. This 10-plate size also includes an OpenArray® Accessories Kit. The TaqMan® OpenArray® Digital PCR Kits are intended for use with our OpenArray® Real-Time PCR System, which enables 12 plates to be run and over 36,000 digital data points to be generated in a single day.

Key product features:
• Accurate and sensitive-Detect and count individual molecules to quantify viral load, gDNA, cDNA, plasmids, or next generation sequencing libraries
• Fast-Produce up to 144 digital answers per 3 hour run
• Flexible-Use your existing assays to test from one to 48 assay/sample dilutions per plate
• Wide dynamic range-By accommodating as few as 64 data points per replicate group, a dilution series can easily be loaded into the TaqMan® OpenArray® Digital PCR Plate, greatly expanding the range of sample concentrations which can be analyzed to produce a digital answer
• Intuitive and economical-Software includes a Poisson calculator to design your individual digital PCR experiment, minimizing optimization time and sample usage

Simple Workflow
TaqMan® OpenArray® Digital PCR Plates are delivered pre-treated with hydrophilic and hydrophobic coatings to accept your own TaqMan® assays. Simply combine the assays with sample and master mix, load onto the TaqMan® OpenArray® plate, seal, cycle, and image. The OpenArray® Real-Time PCR System's simple workflow enables one person to generate over 36,000 digital data points in one day without the use of additional robotics.

Flexible Formats
Each TaqMan® OpenArray® Digital PCR Plate contains 3,072 through-holes arranged in 48 subarrays of 64 through-holes each. The OpenArray® AccuFill™System and open plate format enables the loading of anywhere between one and 48 samples or sample dilutions per plate dependent upon experimental needs.

A Complete Digital PCR Solution
The OpenArray® Digital PCR solution is an affordable, whole-system solution for numerous Digital PCR applications. The decrease in reagent use, afforded by this unique combination of technologies, greatly decreases the price per data point, allowing more samples to be run for a lower cost of consumables. TaqMan® OpenArray® Digital PCR Plates, TaqMan® OpenArray® Digital PCR Master Mix, OpenArray® accessories, and OpenArray® PCR Software have been designed to work together to enable you to obtain accurate and reproducible Digital PCR results.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Contents & storage

This kit contains 10 TaqMan® OpenArray® Digital PCR Plates, TaqMan® OpenArray® Digital PCR Master Mix (5 mL), and an OpenArray® Real-Time PCR Accessories Kit (with accessories for running 10 Plates).


Manuals & protocols