SOLiD™ RNA Barcoding Kit, Module 1-48

Catalog number: 4461565

Applied Biosystems™  Related applications: SOLiD® Next-Generation Sequencing

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Description

The SOLiD™ RNA Barcoding Kit consists of a set of PCR primers that are designed for use with the SOLiD™ Total RNA-Seq Kit or the SOLiD™ SAGE™ Kit with Barcoding Adaptor Module. Together, these combinations of kits enable multiplex sequencing for all RNA applications, including whole transcriptome, small RNA analysis, or SOLiD™ SAGE™.

Key Product Features:

• Cost-effective runs—barcodes enable sequencing of multiple libraries at once
• Modular configuration—each module contains 16 unique barcodes

Increase the Power of Your Studies
Barcoding enables the assignment of a unique identifier to templated beads made from a single library. Once the identifiers are assigned, multiple batches of templated beads may be pooled together for emulsion PCR and then sequenced. The SOLiD™ RNA Barcoding Kit increases the power of your studies by enabling cost effective sequencing of multiple libraries on the same slide—maximizing throughput while dramatically reducing costs. (Figure 1).

Modules of 16 barcodes for RNA Sequencing
The SOLiD™ RNA Barcoding Kit Modules each consist of a set of 16 PCR primers with unique barcodes. There is sufficient material to label 3 libraries with each of the 16 barcodes in each module (96 reactions). The barcodes are selected for uniform melting temperature, low error rate, and orthogonal sequences that are unique in color space.
• The SOLiD™ 5' PCR Primer is identical to the 5’ PCR primer provided in the SOLiD™ Total RNA-Seq Kit and it contains the SOLiD™ emulsion PCR primer P1 sequence.
• The SOLiD™ 3' (reverse) PCR primers contain the P2 sequence required for SOLiD™ emulsion PCR, along with a unique barcode sequence, and an internal adaptor (IA) sequence necessary for sequencing the barcode (Figure 2).

Using these primers in the cDNA amplification step of the procedure generates barcoded cDNA libraries that can be pooled for emulsion PCR and SOLiD™ System sequencing. Additional rounds of ligation-based sequencing are performed using primer sets complimentary to the barcode. The resulting reads can then be sorted by the barcode and aligned in groups to the reference sequence.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): SOLiD™
Library Type: Fragment Library
Workflow Step: Library Barcoding
Starting Material: RNA
Sequencing Type: Transcriptome Sequencing
Product Size: 144 libraries
Final Product: Transcriptome RNA
Downstream Application: Next-Generation Sequencing

Contents & storage

3 x 200 µL SOLiD™ 5' PCR Primer
12 µL each SOLiD™ 3' Primers BC001-048
Store all kit components at –20 °C. Do not store in a frost-free freezer.

Documents

Manuals & protocols