This product will be discontinued on February 28, 2017. Please consider the MagMAX™ FFPE DNA/RNA Ultra Kit as a replacement. View discontinuation letter »

MagMAX™ FFPE DNA Isolation Kit

Catalog number: 4463578

Invitrogen™  Related applications: DNA Extraction

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Description

The MagMAX™ FFPE DNA Isolation Kit delivers faster, cleaner, and easier high-throughput purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. This new product is both deparaffinization-free and xylene-free making for a simpler and safer process. A magnetic bead workflow enables high-throughput scale and speed with yield and purity comparable to its filter-based predecessor Ambion’s RecoverAll™ kit. Whether processed manually, or via automation using the MagMAX™ Express-96 Magnetic Particle Processor, each kit contains all the reagents necessary to process 96 samples, from start to finish in as little as 3 hours.

• Remove xylene and deparaffinization from your FFPE DNA purifications
• Purify DNA from FFPE tissue samples in a high-throughput platform using the kit’s magnetic bead-based technology
• Get DNA in 3 hours (automated) or 3.5 hours (manual)
• Achieve superior performance with our simplified workflow in one complete kit

A Simpler, Faster, and Safer Product
Existing methods utilize xylene or similar solvents followed by alcohol washes to solubilize the paraffin before undergoing a protease digestion and then clean-up with glass fiber filter spin columns. While effective, these methods employ toxic chemicals and require considerable hands-on time. The MagMAX™ FFPE DNA Isolation Kit employs novel chemistries to remove the need for these hazardous solvents by removing the deparaffinization step entirely. A streamlined protocol consisting of solution additions (as opposed to transfers or other steps) consolidates and makes the process simpler, faster, and safer.

Scale and Throughput - Finally
The MagMAX™ FFPE DNA Isolation Kit utilizes magnetic bead chemistry which enables users to scale up to 96 samples at a time. Manual extractions are best performed in a 96-well plate with commonly available magnetic stands and multichannel pipets in approximately 3.5 hours, with approximately 1.5 hours hands-on time. For automated processing, the 3 hour protocol requires only 30 minutes of hands-on time to perform the improved protease-digestion and bead-binding steps, before the sample is placed on the MagMAX™ Express-96 Magnetic Particle Processor for the remaining wash, on-bead nuclease treatment, and elution steps. The process can also be automated on open-format robotics.

Efficient DNA Recovery Without Overnight Digestion
The MagMAX™ FFPE DNA Isolation Kit purifies DNA from up to two 10 µm sections per well with yield comparable to the best-in-class Ambion RecoverAll™ kit and better than competitive offerings. Whereas typical DNA purification requires an overnight digestion step, the MagMAX™ FFPE DNA Isolation Kit requires only a 1.5 hour digestion, significantly reducing the total processing time.

Purified DNA Ready for Downstream Analysis
Recovered DNA is suitable for downstream applications such as PCR, microarray analysis, and mutation screening. However, as is the case with all FFPE tissue, sample fixation and storage typically cause nucleic acid fragmentation and modification. Therefore, downstream applications may require modification for best results.

Complete Kit Convenience
The MagMAX™ FFPE DNA Isolation Kit contains everything needed to perform 96 purifications. RNase, proteinase, magnetic beads, and all wash and elution buffers are supplied. A 96-well processing plate and a 96-well elution plate are also included for convenience.

Need both RNA and DNA from Your FFPE Samples?
A stand-alone MagMAX™ Total Nucleic Acid Isolation Kit is available separately for users who are working with both RNA and DNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): FFPE & Fixed Samples
Sample Type (Specific): Tissue (FFPE)
Isolation Technology: Magnetic Bead
Downstream Application: Next-Generation Sequencing, Northern Blotting, PCR, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Southern Blotting, cDNA Library Construction, microRNA Analysis
Final Product: Genomic DNA
For Use With (Equipment): MagMAX™ Express-96 Deep Well Magnetic Particle Processor
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible, Not High Throughput-Compatible (Manual)
Format: 96-well plate
Elution Volume - min: 70 µl
Number of Reactions: 96 Preps
Product Size: 96 preps
Quantity: 1 kit
Starting Material (Amount): Up to 20 µm section total

Contents & storage

Box 1
Store at Room Temp:
  • Digestion Buffer, 15 mL
  • DNA Digestion Additive, 3 mL
  • Binding Buffer, 40 mL
  • Wash Soln 1 Concentrate, 24 mL
  • Wash Soln 2 Concentrate, 12 mL
  • Elution Buffer, 7 mL
  • Nuclease-Free Water, 18 mL
  • Processing Plate, 1 each
  • Elution Plate, 1 each

Store at 2 to 8°C, Do not freeze:
  • Nucleic Acid Binding Beads, 2 mL

Box 2
Store at -15 to -25°C:
  • Protease, 400 µL
  • RNase A, 100 µL

Documents

Manuals & protocols

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