miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

We have now released the second generation of miRNA inhibitors, mirVana™ miRNA Inhibitors, which:
• Display better potency than lead competitor's product
• Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration
• Are non toxic
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering consistency throughout your entire research project

Positive and negative controls are also available for use along with mirVana™ miRNA Inhibitors in miRNA down-regulation experiments.

mirVana™ miRNA Inhibitor let-7c Positive Control
The mirVana™ miRNA Inhibitor let-7c Positive Control is intended for use as a positive control for mammalian cell culture experiments using mirVana™ miRNA Inhibitors. Endogenous let-7c miRNA has been shown to negatively regulate HMGA2 mRNA in cultured cells. HMGA2 is a ubiquitously expressed, nonhistone, chromatin protein that can modulate gene expression through changes in chromatin architecture. let-7c miRNA down-regulates levels of HMGA2 mRNA. When transfected into human and mouse cell lines, let-7c miRNA inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA. Thus, let-7c miRNA inhibitor activity can be monitored in human or mouse cells using real-time reverse transcription PCR (RT-PCR) to detect HMGA2 mRNA.

First Generation Inhibitor Controls
Our first-generation miRNA inhibitors (and controls), Ambion™ Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Inhibitors and their corresponding controls.