POROS® CaptureSelect® LC Lambda Affinity Column, 2.1 x 30 mm

Catalog number: 4469150

Applied Biosystems™  Related applications: Antibody & Cell Culture Production | Antibody Purification | Chromatography | Protein Purification & Isolation

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Description

Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

Poros® CaptureSelect® LC Lambda HPLC columns incorporate the advantages of high-speed Poros® chromatography resins with the unique selectivity of CaptureSelect® ligands. The combination allows three minute quantitation of Fabs, immunoglobulins, and bispecific antibodies having a Lambda light chain.

Features:

• Broad utility—POROS® A, G and CaptureSelect®affinity ligands address most biological drug classes
• Rapid—POROS® resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—deliver high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect® LC Lambda affinity ligands when combined with POROS® 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of all immunoglobulins and Fabs having a Lambda light chain (all subclasses).

POROS® perfusion chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.

Automation
POROS® affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS® Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

LC Lambda Column Specifications:
Protein Binding Capacity 10 mg/mL
Species Specifiity Human
Column Material PEEK™ polymer or stainless steel
Frit 2 µm, titanium
POROS® Resin Cross-linked poly (styrenedivinylbenzene)
CaptureSelect® Ligands Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae


For Research Use Only. Not for use in diagnostics procedures.

Specifications

Format: Column
Flow Rate: Up to 5000 cm⁄hr
Pore Size: 0.16 micron
Dimensions: 2.1 mm (diameter) x 30 mm (length)
Reactivity: Human
Column Type: Affinity Column, PrePacked Column
Product Size: 1 column
Particle Size: 20 micron
Support Matrix: Polystyrenedivinylbenzene
Target Molecule: Lambda Light Chain
Volume (Metric): 100 µl column volume
Binding Strength: 10.0 mg/ml dynamic binding capacity
Immobilized Phase: CaptureSelect™ LC Lambda
Shipping Condition: Wet Ice
Elution Volume - max: 200 µl
Elution Volume - min: 100 µl
Sample Type (Specific): Cell Culture Supernatants
For Use With (Equipment): HPLC
Maximum Operating Pressure: 180 bar
High Throughput Compatibility: Not High Throughput-Compatible (Manual)

Contents & storage

Column includes EZ grip fittings and column test certificate



Store at 4°C DO NOT FREEZE

Documents

Manuals & protocols