AmpFLSTR® Identifiler® Direct PCR Amplification Kit & Prep-n- Go™ Buffer, for buccal swabs

Catalog number: 4473287

Applied Biosystems™  Related applications: DNA Extraction | PCR Amplification for Human Identification | Sample Prep⁄Extraction for Human Identification

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Description

The AmpFLSTR® Identifiler® Direct PCR Amplification Kit & Prep-n-Go™ Buffer (for Buccal Swabs) combines the features of our AmpFLSTR® Identifiler® Direct PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer (for Buccal Swabs). The Identifiler® Direct kit enables direct PCR amplification of the same 16 loci included in our standard Identifiler® kit, while eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Prep-n-Go™ Buffer (for Buccal Swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key Features:

• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction?purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler® Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Identifiler® kit, which:

• Maximizes concordance with STR profiles generated with other AmpFLSTR® kits including the Identifiler®, Identifiler® Plus, Profiler Plus®, Profiler Plus® ID, SGM Plus® and COfiler® Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For Forensic or Paternity Use Only.

Specifications

Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, VIC®, LIZ™, PET™, FAM™
Product Size: 200 reactions
Amplicon Size: <360 bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): Forensic Sample, Buccal Swab
For Use With (Equipment): 3500xL Genetic Analyzer, 3500 Genetic Analyzer, GeneAmp® 9600, 3730 DNA Analyzer, 3130xl Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130 Genetic Analyzer
Matrix and Size Standards: Matrix Standard Set DS-33, GeneScan™ 500 and 600 LIZ® Size Standards

Contents & storage

AmpFLSTR® Identifiler® Direct Master Mix
AmpFLSTR® Identifiler® Direct Primer Set
AmpFLSTR® Identifiler® Direct Control DNA 9947A
AmpFLSTR® Identifiler® Direct Allelic Ladder
Store at -20 °C upon receipt, 2 to 8 °C after initial use

1 Prep-n-Go™ Buffer Bottle (80 mL)
200 tests at 400 µL/test
Store at room temperature and protect from light

Documents

Manuals & protocols

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