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Ion PGM™ Template OT2 200 Kit

Catalog number: 4480974

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

The Ion PGM™ Template OT2 200 Kit contains template preparation reagents for automated and reproducible template preparation for 200 base-read libraries using the Ion OneTouch™ 2 System. As an integral component of the semiconductor sequencing workflow, the Ion PGM™ Template OT2 200 Kit is designed to make high-quality templated Ion Sphere™ particles using the Ion OneTouch™ 2 System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery.

The Ion PGM™ Template OT2 200 Kit provides you with:

• Simple template preparation for 200 base-read libraries
• Support for Ion 314™, Ion 316™, and Ion 318™ chips
• Automation to obtain high-quality sequencing results every time
• Multiplexed library support

Single Day Sequencing Workflow
Ion Torrent revolutionizes template preparation by integrating 4 workflows into a single system: 1) microreactor generation for massively parallel clonal amplification, 2) template amplification using a novel in-line PCR technology, 3) Ion Sphere™ particle recovery using an integrated mini-centrifugation system, and 4) template-positive Ion Sphere™ particle enrichment by magnetic separation. By integrating multiple workflows, the Ion OneTouch™ 2 System and associated reagent kit shorten template preparation to minutes of hands-on time, allowing you to progress from sample to results in a single day. The modular nature of the Ion sequencing workflow allows processing of up to 2 samples per day.

Increased Productivity and Lower Costs
The Ion OneTouch™ 2 System and this reagent kit support the Ion 314™, Ion 316™, and Ion 318™ chips, for sequence throughputs up to 1 Gb. The modular nature of the system facilitates QC testing of your sample to assess Ion Sphere™ template positivity and yield pre- and post-enrichment for greater peace of mind regarding the likelihood of sequencing success with your sample. The system also drastically reduces time and cost by supporting template preparation of multiplexed libraries for DNA-based applications.

The Ion PGM™ Template OT2 200 Kit contains template preparation reagents for sequencing 10 samples using the Ion OneTouch™ 2 System. The kit components include proprietary Ion Sphere™ particles, buffers, PCR reagent, and enzyme mixes, consumables, and primers.

Notes:
This kit is only compatible with the Ion OneTouch™ 2 System.
This kit is not compatible with the Ultra Turrax Drive from IKA® for the Ion PGM™ System or the Ion OneTouch™ System.
This kit supports 200 bp run configuration.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion PGM™ System
Product Size: 10 reactions
Workflow Step: Template Preparation, Sample Enrichment
Chip Compatibility: Ion 314 Chip, Ion 316 Chip, Ion 318 Chip
High Throughput Compatibility: Automated Protocols, Multiplexing, High Throughput-Compatible

Contents & storage

The Ion PGM™ Template OT2 200 Kit contains template preparation reagents for sequencing 10 samples using the Ion OneTouch™ 2 System.

Kit components include proprietary Ion Sphere™ particles, buffers, PCR reagent and enzyme mixes, consumables, and primers.

Store components at -20 °C, 4 °C, or room temperature, as appropriate.

Documents

Manuals & protocols