Ion Library Equalizer™ Kit

Catalog number: 4482298

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.

The kit workflow consists of three simple steps:

1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer

Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer®-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability

Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer®-based workflow (up to 10 samples)
• Reduced AMPure® XP reagent usage compared to Agilent 2100 Bioanalyzer®-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency

Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization

Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.

Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit

Optional:
Ion Xpress™ Barcode Adapters Kits
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion PGM™ System, Ion Proton™ System
Library Type: Fragment Library, Normalized Library, Targeted Sequencing Library
Workflow Step: Library Generation, Library Barcoding, Template Preparation
Starting Material: DNA
Sequencing Type: Epigenetic Sequencing, Genome & DNA Sequencing
Species: All Species
Technique: Amplicon Sequencing
Product Size: 96 reactions
Multiplex Capability: No Limit on Multiplexing
Sample Type (Specific): Any Sample Type
Starting Material (Amount): Fragment or Targeted AmpliSeq Libraries

Contents & storage

1 tube Equalizer Primers - Store at 2-8 °C
1 tube of Equalizer Capture - Store at 2-8 °C
1 tube of Equalizer Wash Buffer - Store at 2-8 °C
1 tube of Equalizer Elution Buffer - Store at 2-8 °C
1 tube of Equalizer Beads - Store at 2-8 °C

Note kits are shipped at ambient temperature store each component at specified temperatures.

Documents

Manuals & protocols

Recommended products