Ion Torrent™

Ion AmpliSeq™ RNA Cancer Panel

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The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes.

• Measure gene expression fold changes between samples
• Amplicons are designed to be most inclusive for transcripts of the target gene
• Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA
• Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system

This panel contains 50 amplicons (100 primers), approximately 150 bases in length, in a single pool.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System
Library Type: Targeted Sequencing Library
Workflow Step: Target Selection
Starting Material: Total RNA
Sequencing Type: Transcriptome Sequencing
Product Line: Ion AmpliSeq™
Product Size: 24 reactions
Sample Type (Specific): RNA (>500 bases), Tissue (FFPE)
Species: Human
Starting Material (Amount): 500 pg of total RNA from fresh frozen tissue or 5 ng of RNA from FFPE samples
Technique: Amplicon Sequencing

Contents & storage

1 tube 5X RNA Cancer Panel - Storage: -30 to -10 °C


Manuals & protocols