This kit along with the Ion PGM Controls Kit (SKU# 4480449) make up the Ion PGM™ IC 200 Starter Kit (SKU# 4488812). We highly recommended purchasing both kits together for training and start of project.

Ion PGM™ IC 200 Kit

Catalog number: 4484080

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

The Ion PGM™ IC 200 Kit enables robust and reproducible template preparation, chip loading, and sequencing of 200 base-read libraries on the Ion Chef™ system for the Ion PGM™ system sequencing workflow. The plug & play, pre-packaged single-use reagent cartridges for template and chip preparation provide a fully automated workflow, processing dual samples per run for increased productivity. The reagent and sample barcoding features interact with the Ion Chef™ system to validate user-defined run plan and offer complete run traceability.

The Ion PGM™ IC 200 Kit offers:

• Fully automated and reproducible template preparation, chip loading, and sequencing of 200 base-read libraries
• Less than 15 minutes hands-on time
• Ready-to-use, disposable reagent cartridges
• Increased throughput with dual sample processing
• Multiplexing capability
• Increased robustness for quality sequencing results

The Ion PGM™ IC 200 Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow, designed to simplify and streamline the overall workflow with minimal hands-on-time for users with any experience level. This easy-to-use kit delivers high-quality and reproducible templated products for semiconductor sequencing.

The Ion PGM™ IC 200 Kit contains sufficient reagents and consumables for 8 template preparation and sequencing runs, and should be used in conjunction with Ion 314™, Ion 316™, or Ion 318™ v2 barcoded chips for sample tracking purposes.

Notes:

• This kit supports 200 base-read libraries for a variety of applications.
• This kit is only compatible with the Ion Chef™ system and the Ion PGM™ system.
• Components of this kit have been optimized for use on the Ion Chef™ system and the Ion PGM™ system specifically; and should NOT be replaced or interchanged with other product kit components.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion PGM™ System, Ion Chef™ System
Library Type: 200 base-read libraries
Workflow Step: Template Preparation, Chip Loading, Sequencing
Sequencing Type: Genome & DNA Sequencing
Product Size: 8 reactions
Chip Compatibility: Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, Ion 318™ Chip v2 BC
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible, Multiplexing

Contents & storage

The Ion PGM™ IC 200 Kit components include template reagents, enzyme mixes, nucleotides, spike-in sequencing controls, solutions & buffers, consumables, and supplies.

Documents

Manuals & protocols

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