This product has been replaced by the Ion PI™ Sequencing 200 Kit v3, which delivers enhanced sequencing quality and coverage for Ion™ Proton applications.

Ion PI™ Sequencing 200 Kit v2

Catalog number: 4485149

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

The Ion PI™ Sequencing 200 Kit v2 contains reagents and consumables for running the single-day sequencing workflow on the Ion Proton™ Sequencer. As an integral component of the Ion Proton™ sequencing platform, the Ion PI™ Sequencing 200 Kit v2 is designed to sequence templated Ion PI™ Ion Sphere™ particles deposited in Ion PI™ chips for fast human exome resequencing or transcriptional profiling.

The Ion PI™ Sequencing 200 Kit v2 provides you with:

• Support for PI™ chips, enabling up to 80 million reads per run
• Sequencing throughputs of 10 Gb or greater with 200 base read lengths
• Low cost per run for a variety of applications, including exome and whole transcriptome sequencing

Rapid and Comprehensive Genomic Profiling
The Ion Proton™ workflow along with TargetSeq™ technology and Ion Reporter™ Software enables comprehensive exome sequencing on your benchtop. Delivering up to 80 million reads in a single run, the Ion Proton™ System, coupled with Ambion® RNA kits and Partek® Flow® Software, provides transcriptome sequencing on demand.

Single-Day Sequencing Workflow
The fast sequencing and data processing times of the Ion Proton™ System enable sample to results in a single day. With such rapid turnaround times, the Ion Proton™ System aims to be approximately three times faster than the projected performance of other next-generation sequencing platforms.

Increased Productivity and Lower Costs
The new Ion PI™ Sequencing 200 Kit v2 provides sequencing reagents for high throughput semiconductor-based sequencing of DNA- and RNA-based libraries. This new kit supports Ion PI™ chips and yields higher accuracy and higher throughputs per unit time than other next-generation sequencing platforms. This economically priced kit offers lower sample preparation costs, bringing next generation sequencing to every lab.

The Ion PI™ Sequencing 200 Kit v2 contains reagents for 8 sequencing reactions using the Ion Proton™ System. Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents.

Notes:
This kit is only compatible with the Ion Proton™ System.
Use this kit with the Ion PI™ Template OT2 200 Kit v2 and Ion PI™ Chip Kit v2.
This kit replaces the Ion PI™ Sequencing 200 Kit & the Ion PI™ Chip Preparation Kit.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion Proton™ System
Recommended Applications: Resequencing
Technique: Post-Light™ Ion Semiconductor Sequencing
Read Length: Up to 200 bases
Product Size: 8 reactions
Chip Compatibility: Ion Proton I Chip
High Throughput Compatibility: High Throughput-Compatible, Multiplexing

Contents & storage

Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents.

Store components at -5 to -30°C, 4 °C, or room temperature as indicated.

Documents

Manuals & protocols