BigDye® Direct Cycle Sequencing Reagents-CG

Catalog number: 4486191

Applied Biosystems™  Related applications: DNA Sequencing | Diagnostic Development | Sanger Sequencing

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The BigDye® Direct Cycle Sequencing Reagents-CG are General Purposes Reagents that combine PCR purification and cycle sequencing, enabling a streamlined protocol and shorter time to results. With these reagents, all steps from PCR to loading on the capillary can be performed in the same reaction tube, minimizing the number of manual handling steps and the possibility of experimental variability. These reagents are for use with capillary electrophoresis-based genetic analyzers and are not validated for use with other systems or applications. These reagents are For Laboratory Use.

Features of the BigDye® Direct Cycle Sequencing Reagents include:

• Streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the first base after the gene-specific primer sequence
• Substantial time savings versus previous workflows

Save Time and Get Better Results with a Unique Streamlined Workflow
The BigDye® Direct Cycle Sequencing Reagents have been developed to minimize complexity and hands-on time. Compared with previous sequencing procedures, which typically require 5 processing steps and take up to 8 hours, Big Dye® Direct reagents can deliver results in 4 steps taking approximately 5 hours—up to a 40% time savings.

These reagents provide sufficient reagents for two key steps of the sequencing workflow:

• The PCR reaction—using the BigDye® Direct PCR Master Mix and M13 tailed primer (not provided); PCR is performed in approximately 1 hour.
• The unique one-step PCR purification and cycle sequencing reaction—using the BigDye® Direct Sequencing Master Mix and the BigDye® Direct M13 primers provided. (M13 tailed primer used for the PCR reaction simplifies the sequencing reaction setup by using two sequencing primers independently of the PCR product analyzed.)

An Integrated Solution Ideal for Resequencing
The BigDye® Direct Cycle Sequencing Reagents are the first product designed to maintain conditions leading to high quality data generation throughout the entire read length with faster run times compared to common sequencing workflows. This is achieved through a unique reagent formulation that allows high-resolution data reads closer to the primer and the use of POP-7™ polymer for faster capillary electrophoresis times.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): 3730 DNA Analyzer, 3130 Genetic Analyzer, 3500 Genetic Analyzer
Recommended Applications: Sequencing
Product Size: 100 reactions

Contents & storage

• BigDye® Direct M13 Forward Primer-CG
• BigDye® Direct M13 Reverse Primer-CG
• BigDye® Direct PCR Master Mix-CG
• BigDye® Direct Sequencing Master Mix-CG
• CEPH 1347-02 Control DNA-CG, 50 ng/µL


Manuals & protocols