BigDye® Terminator v1.1 Cycle Sequencing Reagents-CG

Catalog number: 4486480

Applied Biosystems™  Related applications: Diagnostic Development | Sanger Sequencing

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The BigDye Terminator v1.1 Cycle Sequencing Reagents-CG are General Purpose Reagents that provide the required components for cycle sequencing reactions in a pre-mixed format. You need only provide the template and template-specific primer. These reagents are suitable for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA templates, PCR fragments, and large templates (e.g., BAC clones). These reagents are for use with capillary electrophoresis-based genetic analyzers and are not validated for use with other systems or applications. These reagents are For Laboratory Use.

This BigDye® Terminator v1.1 Cycle Sequencing Reagents are designed for specialty applications that require optimal base-calling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Sequence challenging templates and sequences more successfully
• Obtain longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Tackle Tough Sequences
With the BigDye® Terminator v1.1 Cycle Sequencing Reagents you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

More Uniform Peak Heights, Improved Accuracy
The BigDye® Terminator v1.1 reagents' superior chemistry helps you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Laboratory Use


For Use With (Equipment): 3730 DNA Analyzer, 3130 Genetic Analyzer, 3500 Genetic Analyzer
Recommended Applications: Sequencing
Product Size: 100 reactions

Contents & storage

• M13(-21) Primer-CG
• pGEM® 3Zf(+)-CG
• BigDye® Terminator v1.1 Cycle Sequencing RR Mix-CG
• 5x Sequencing Buffer-CG


Manuals & protocols