Ion AmpliSeq™ Exome Kit 8xTrio

Catalog number: 4489839

Ion Torrent™  Related applications: DNA Sequencing | Ion Torrent™ Next-Generation Sequencing

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Description

The Ion AmpliSeq™ Exome Kit 8xTrio enables the fastest and simplest method of exome enrichment to accelerate your human disease research, and is intended for use with the Ion PI™ Template OT2 200 Kit v3 and Ion PI™ Sequencing 200 Kit v3. Producing exome libraries in under 6 hours with less than 60 minutes of hands-on time and leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq™ technology along with the Ion Proton™ Sequencer, the Ion AmpliSeq™ Exome Kit 4xDuo allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.

The Ion AmpliSeq™ Exome Kit 8xTrio includes the following components for 24 exomes (running 3 exomes per chip):

• Ion AmpliSeq™ Exome Panel: oligo pools/primers for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq™ Library kits for preparation of amplicon libraries using Ion AmpliSeq™ technology
• Ion PI™ Chip Kit v2 for sequencing on the Ion Proton™ Sequencer

An Ion PI™ Reagents XT Kit is not included in this version of the kit since the latest (v3) template and sequencing kits no longer require the use of these reagents.

The Ion AmpliSeq™ Exome Kit 8xTrio along with the Ion Proton™ System enable you to:

Simplify exome enrichment through the combination of PCR and the Ion AmpliSeq™ workflow, resulting in less than 1 hour of hands-on time to produce exome libraries in under 6 hours
Accelerate your disease research with rapid exome sequencing, going from DNA to variants in just 2 days
Access affordable exome sequencing when you need it, without the need to batch dozens of samples or the high price of a lower-throughput run
Maximize sequencing efficiency with >90% on-target bases and >92% coverage uniformity, and design covering >97% of the Consensus Coding Sequences (CCDS) annotation
Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite™ and Ion Reporter™ software

Quickest Exome Enrichment with Unprecedented Simplicity
The Ion AmpliSeq™ Exome Kit 8xTrio provides unmatched simplicity for exome enrichment. With a workflow requiring less than 60 minutes of hands-on time and no centrifugation steps, the kit enables exome libraries in less than 6 hours with the least amount of hands-on time. Additionally, the Ion AmpliSeq™ Exome Kit 8xTrio maintains a low input requirement of 50 ng DNA.

Lowest Cost per Exome Using a Benchtop Sequencer
Paired with the Ion Proton™ System, the Ion AmpliSeq™ Exome Kit 8xTrio allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion AmpliSeq™ Exome Kit 8xTrio with the Ion Proton™ System enables you to go from DNA to variants in as little as 2 days. Additionally, with the most uniform exome enrichment covering >97% of CCDS, you can achieve the most efficient sequencing with 2 exomes run per Ion PI™ chip.

Fully Integrated Analysis without Bioinformatics Expertise
The combination of Torrent Suite™ and Ion Reporter™ software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion Proton™ Sequencer along with Ion Reporter™ Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion Proton™ System
Library Type: Targeted Sequencing Library
Workflow Step: Sample Enrichment, Target Selection
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Size: 24 exomes
Final Product: PCR Amplicons

Contents & storage

Contains:

• 9 Ion AmpliSeq™ Library Kits 2.0 (store at -5 to -30°C)
• 3 Ion AmpliSeq™ Exome Panel (store at -5 to -30°C)
• 2 Ion PI™ Chip Kit v2 (4-pack) (store at room temperature)

Documents

Manuals & protocols