Dynabeads® Streptavidin Trial Kit

Catalog number: 65801D

Invitrogen™  Related applications: DNA Extraction | Protein Assays and Analysis | Protein Purification & Isolation | Viral RNA⁄DNA Purification

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Description

Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated molecules and targets (e.g. antibodies, proteins, peptides, small molecules, sugars, lectins, oligonucleotides and DNA/RNA).

The beads are available with different sizes and surface properties, This low-cost Trial Kit allows you to test the flexible Dynabeads® in your application

Applications:
The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is utilized in a vast number of applications. Over the past 15 years, Streptavidin-coupled Dynabeads® have been widely used and cited. Examples include direct/indirect capture, separation and downstream handling of nucleic acids, purification of DNA/RNA binding proteins, proteins/peptides, as well as protein interaction studies, immunoprecipitation, immunoassays, phage display, biopanning, drug screening and cell isolation. Did you know that Dynabeads® are employed on more than 25,000 routine IVD instruments worldwide?

Benefits and Features:

Direct and fast isolation of any biotinylated molecule

• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• No need for filters, columns or centrifugations
• Manual or automated - protocols can easily be adjusted from manual to automated use on any laboratory robot that can be equipped with a magnet
• Rapid liquid-phase kinetics give efficient capture with a high signal-to-noise ratio and increased sensitivity
• High batch-to-batch reproducibility, securing consistent results in your application

The uniform and superparamagnetic Dynabeads® Steptavidin have a monolayer - not a multilayer - of recombinant streptavidin covalently coupled to their surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. Their specific and defined surface allow for efficient capture, separation and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency.

The product holds reputable Dynal high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

More Product Information:
Please refer to the product descriptions for the 4 different bead-types in this trial kit: Dynabeads® M-280 Streptavidin, Dynabeads® M-270 Streptavidin, Dynabeads® MyOne™ Streptavidin C1 and Dynabeads® MyOne™ Streptavidin T1. See Product Systems at right of this page for other Dynabeads® Streptavidin products
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Ligand Type: Streptavidin
Form: Beads in Suspension
Shelf Life: 36 months from date of manufacture
Product Size: 4 x 1 mL
Bead Diameter: 1 µm, 2.8 µm
Concentration: 10 mg⁄ml
Binding Property: 650->2,500 pmoles/mg beads
Isoelectric Point: pH 4.5 - 5.2
Size Distribution: CV < 3%
Surface Functionality: Hydrophobic, Carboxylic Acid, Hydrophilic, Tosylactivated
Sample Type (Specific): Any Sample Type
Iron Content (Ferrites): 12% - 26%
High Throughput Compatibility: High Throughput-Compatible
Shipping Condition: Room Temperature

Contents & storage

This Product Contains: The Trial Kit has been created so you can test each of these 4 streptavidin-coated Dynabeads® to find the best match for your application. The kit contains 1 ml (10 mg/ml) of each of the following bead-types: Dynabeads® M-280 Streptavidin (2.8µm beads supplied at a concentration of 6-7x10^8 beads per ml, Dynabeads® M-270 Streptavidin (2.8µm beads supplied at a concentration of 6-7x10^8 beads per ml, Dynabeads® MyOne™ Streptavidin C1 (1 µm beads supplied at a concentration of 7-12x10^9 beads per ml, Dynabeads® MyOne™ Streptavidin T1 (1 µm beads supplied at a concentration of 7-12x10^9 beads per ml
Store at: 2-8°C

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Manuals & protocols

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