SuperPicture™ 3rd Gen IHC Detection Kit

Catalog number: 878973

 Related applications: Anatomical Pathology

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Description

The SuperPicture™ polymer detection kit features Invitrogen’s proprietary HRP polymer technology to provide excellent sensitivity, high specificity, and a shorter protocol than conventional immunohistochemical staining methods. Invitrogen’s new polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. Drawbacks of other polymer systems include compromised cell penetration ability that can cause weak nuclear staining. Invitrogen’s polymer conjugate contains multiple HRP molecules to provide excellent sensitivity and specificity. SuperPicture™’s staining method is faster and easier than a conventional LAB-SA protocol because it uses an HRP polymer conjugated to the secondary antibody and eliminates one incubation step. In addition, SuperPicture™ does not use biotin, so potential background due to endogenous biotin activity is completely avoided. SuperPicture™ also features a shorter polymer incubation time; SuperPicture™ only requires 10 minutes, while leading competitors’ polymers require 30 minutes.

This new and improved SuperPicture™ 3rd Gen IHC Detection Kit features improved DAB components for greater signal sensitivity and dependable performance. The enhanced DAB in this 3rd Gen kit also allows for greater consistency and stability when mixed, and results in excellent IHC stains with minimal background.
For In Vitro Diagnostic Use.

Specifications

Substrate: DAB (Enhanced)
Technique: HRP Polymer
Reactivity: Mouse, Rabbit, Rat, Guinea Pig
Host Species: Goat
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 1,000 slides
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Target Species: Human
Detection Method: Colorimetric
Target Specificity: Not Target-Specific
Number of Reactions: 1000 Reactions
For Use With (Equipment): Light Microscope
Shipping Condition: Wet Ice

Contents & storage

This kit contains sufficient reagents to stain 1000 slides.

Store kit at 2-8°C. All performance claims are void after kit expiration date.

Reagent A. Peroxidase Quenching Solution (110 ml)
Reagent B. HRP Polymer Conjugate (110 ml)
Reagent C1. DAB Substrate Buffer (110 ml)
Reagent C2. DAB Chromogen (3.5 ml)

Documents

Manuals & protocols