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PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY® FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate)

Catalog number: A10070

Molecular Probes™  Related applications: Protein Assays and Analysis

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Description

Molecular Probes® phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Fluorogenic Phospholipase A Substrates Specifications:

• Label (Ex/Em): PED-A1
• Specificity: PLA1
• Cleavage product: D3834
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry

Find More Probes for Lipid Metabolism and Fatty Acid Analogs
Review Fatty Acid Analogs and Phospholipids—Section 13.2 and Probes for Lipid Metabolism and Signaling—Section 17.4 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: Phospholipase A1 selective substrate
Product Size: 100 µg
Target Enzyme: Phospholipase
Substrate Type: Phospholipase Substrate
Detection Method: Fluorescent
Cell Permeability: Cell-Permeant
Substrate Properties: Lipid-Based Substrate
For Use With (Equipment): Microplate Reader, Flow Cytometer
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols