RPMI 1640 Medium (ATCC modification)

Catalog number: A1049101

Gibco™  Related applications: Mammalian Cell Culture

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Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With
L-glutamine
HEPES
Phenol Red
Sodium Pyruvate
High glucose
Low sodium bicarbonate

The complete formulation is available.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has been modified by ATCC to contain high glucose, low sodium bicarbonate, sodium pyruvate, HEPES, and low L-glutamine. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (1.5 g/L) and therefore requires a 5–7% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: HEPES
Serum Supplementation: Standard Serum Supplementation
Sodium Pyruvate Additive: Sodium Pyruvate
Glucose: High Glucose
Product Size: 500 mL
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Documents

Manuals & protocols

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