SequalPrep™ Long PCR Kit with dNTPs

Catalog number: A10498

Applied Biosystems™  Related applications: DNA Sequencing | PCR | SOLiD® Next-Generation Sequencing | Sanger Sequencing

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Description

The SequalPrep™ Long PCR Kit with dNTPs provides the highest amplification success rate of any long range PCR kit tested across multiple organisms and platforms for non-optimized amplicons up to 20 kB with little to no optimization. Successful long range PCR with minimal optimization enables efficient PCR enrichment for a variety of applications including medical re-sequencing on next generation sequencing (NGS) platforms.
PCR amplification is the traditional method to enrich target regions of the genome for genotyping and re-sequencing applications. To take advantage of the enhanced NGS processing capabilities, higher throughput enrichment methods have become necessary. Unfortunately, the potential efficiency gains from long range PCR using available commercial solutions have been offset with lower amplification success rates limiting enrichment to ~5 kB regions. The SequalPrep™ Long PCR Kit with dNTPs overcomes these issues and provides the highest amplification success rate of any commercially available kit tested across multiple organisms and platforms for amplicons up to 20 kB with minimal optimization.

This kit can be used upstream of any NGS platform or for other genotyping applications. When combined with the SequalPrep™ Normalization Plate the SequalPrep™ (Sequence Equalization Sample Preparation) System is the first complete PCR enrichment and amplicon equalization solution that provides a viable and efficient enrichment method to meet NGS re-sequencing throughput capabilities. This NGS Sample Prep system significantly reduces PCR amplification and normalization complexity to allow for enrichment of regions up to 1MB in a 96 well plate format. Amplify and equalize with the ease of SequalPrep™ for consistently high coverage of your regions of interest.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: SequalPrep™ Long Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 4 X
GC-Rich PCR Performance: Medium
Reaction Format: Separate Components
Product Overhang: Mixed
Reaction Speed: Standard
Final Product Size(s): 20 kb or less
Product Size: 1,000 units
Exonuclease Activity: 5' - 3', 3' - 5'
Shipping Condition: Dry Ice

Contents & storage

• 200 µl SequalPrep™ Long Polymerase (5 U/µl)
• 1.2 ml SequalPrep™ 10X Reaction Buffer
• 1.2 ml SequalPrep™ 10X Enhancer A
• 1.2 ml SequalPrep™ 10X Enhancer B
• 750 µl DMSO

Store at -20°C. DMSO may be stored at 4°C or room temperature.

Documents

Manuals & protocols

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