MembraneMax™ Protein Expression Kit

Catalog number: A10633

Invitrogen™  Related applications: Protein Expression

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Description

MembraneMax™ Protein Expression Kits delivers high yields of soluble (dispersed) membrane proteins using the MembraneMax™ reagent, which is an planar phospholipid membrane bilayer surrounded by a scaffold protein (also called a nanolipoprotein particle or NLP). Combining this technology with a scalable cell-free expression E. coli extract delivers microgram to milligram quantities of your membrane protein specifically for high throughput expression screening, expression of membrane proteins that would otherwise be toxic in cell-based systems and functional or activity assays. With high uniformity and consistent structure, MembraneMax reagent minimizes formation of membrane protein aggregates or clumping often caused by inconsistent size, shape and structure typical observed with microsomes or micelles. The MembraneMax™ Protein Expression kits contain all the necessary reagents for successful expression of your membrane protein.
Key Advantages of the MembraneMax™ Protein Expression Kits:
• Optimized cell-free expression delivers high yield of membrane protein from nanogram to milligram quantities
• MembraneMax™ reagent enables a monodispersed population of soluble membrane protein-NLP complexes
• Expression format amenable to high-throughput protein synthesis for screening and expression of toxic proteins
• Production of a homogeneous population of your membrane protein
• Convenient kit format includes the necessary reagents for protein expression—simply add your gene of interest to get started
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: T7
Expression: Cell-Free
Product Size: 100 reactions
Expression Mechanism: Cell-Free Expression
Protein Tag or Fusion: Untagged
Expressed Protein Type: Membrane Protein
High Throughput Compatibility: Not High Throughput-Compatible (Manual)

Contents & storage

Expressway™ Maxi Expression Module
Expressway™ Maxi Amino Acids Module
MembraneMax™ Expression module
Expressway™ (Extract) Module: Store at -80°C
Expressway™ Amino Acids module and MembraneMax™ Expression modules: Store at -20°C

Documents

Manuals & protocols

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