This product has been superseded by the RiboMinus™ Eukaryote System v2 and Low Input RiboMinus™ Eukaryote Kit v2 and will be discontinued in the near future.

RiboMinus™ Eukaryote Kit for RNA-Seq

Catalog number: A1083708

Ambion™  Related applications: RNA Extraction | RNA Sequencing | SOLiD® Next-Generation Sequencing

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The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:

• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module.
For Research Use Only. Not for use in diagnostic procedures.


System: RiboMinus
Quantity: 1 each
Product Size: 8 preps
Final Product: Transcriptome RNA
Yield (up to): 1 µg
Sequencing Type: Transcriptome Sequencing
Isolation Technology: Magnetic Bead
Sample Type (General): RNA
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, Microarray Analysis, Sequencing, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), cDNA Library Construction
Sample Type (Specific): Total RNA
Starting Material (Amount): 2-10 µg Total RNA
Starting Material (Volume): 20 µl
High Throughput Compatibility: High Throughput-Compatible
Shipping Condition: Wet Ice

Contents & storage

Each kit contains:
• 6 ml RiboMinus™ Magnetic Beads
• 80 µl RiboMinus™ Eukaryote Probe (15 pmol/µl)
• 10 ml Hybridization Buffer
• 12 ml DEPC-treated (RNase-free) water
Store at 4°C.


Manuals & protocols

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