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Biocytin Alexa Fluor® 488 (Alexa Fluor® 488 Biocytin, Disodium Salt)

Catalog number: A12924

Molecular Probes™  Related applications: Cell Tracing & Tracking

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Description

The cell-impermeant, fixable, polar tracer Alexa Fluor® 488 biocytin combines the green-fluorescent Alexa Fluor® 488 fluorophore with biotin and an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The biotin allows for detection and subsequent amplification with enzyme-modified streptavidins including horseradish (HRP) and alkaline phosphatase (AP) that can be used with TSA and ELF technologies, respectively.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Biocytins
Product Size: 250 µg
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.

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