KnockOut™ ESC/iPSC Media Kit

Catalog number: A1413001

Gibco™  Related applications: Stem Cell Culture

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Description

This bundle provides a single kit to build the ideal media system for feeder-based culture of pluripotent stem cells. The kit includes KnockOut™ SR, DMEM/F12 with GlutaMAX™ supplement, and FGF - just combine according to the protocol, add the NEAA and BME, and you are ready to go!

The KnockOut™ ESC/iPSC Medium Kit is:

• The perfect start to your feeder-based pluripotent stem cell culture
• Easy to order: order your base media with one simple catalog number
• Cost effective

Get Started with our Best Serum-Free System for Stem Cell Culture
Start your feeder-based stem cell culture right here with the most referenced product in the literature - KnockOut™ Serum Replacement - then combine with DMEM/F12 with GlutaMAX™ supplement and FGF. Knockout™ Serum Replacement (Knockout™ SR) is a defined, serum-free formulation optimized to grow and maintain undifferentiated ES cells in culture. It directly replaces FBS in existing protocols.

Save Money and Time by Buying Your Stem Cell Culture Basics as a Kit
The KnockOut™ ESC/iPSC Medium Kit provides you with a cost-effective way to put together your pluripotent stem cell feeder system. With one catalog number you can easily order your cell culture basics, and the enclosed protocol will provide all the guidance needed to get your cultures up and going.

Part of the Life Technologies Family of Stem Cell Culture Products
Life Technologies offers a broad array of innovative and widely published products for embryonic and induced pluripotent stem cell culture. You can trust Life Technologies for consistent high quality and professional support.

Learn More About Products for Embryonic Stem Cell Research

Learn More About Products for Induced Pluripotent Stem Research

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Stem Cells, Stem Cells (Embryonic), Stem Cells (iPS - Induced Pluripotent Stem), Stem Cells (Mouse), Stem Cells (Human)
Glutamine: GlutaMAX™-I
Serum Level: Serum-Free
HEPES Buffer: No HEPES
Product Size: 5 x 500 mL
Volume (Metric): 2.5 L
Included Antibiotics: No Antibiotics
Phenol Red Indicator: Phenol Red
Sodium Pyruvate Additive: Sodium Pyruvate

Contents & storage

KnockOut™ ESC/iPSC Media Kit includes:
  • 5 X 500 mL DMEM/F12 with GlutaMAX (SKU# 10565018)
  • 5 X 100mL of KSR (SKU# 10828010)
  • 2 X 10ug of FGF (SKU# PHG0264)
Storage:
  • DMEM/F12 with GlutaMAX — 2-8°C
  • KSR: -20°C
  • FGF: -20°C

Documents

Manuals & protocols