pJTI™ R4 Exp CMV EmGFP pA Vector

Catalog number: A14146

 Related applications: Cell Line Development for Protein Production | Protein Expression

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Description

The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting reaction with a Jump-In™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector) included in the Jump-In™ parental kits, the EmGFP will be expressed and positive cells will fluoresce green.

Ensure the Success of Your Jump-In™ Retargeting Reactions
Successful retargeting of Jump-In™ parental cell lines like the the Jump-In™ GripTite™ HEK293 kit (A14150) is dependent on a variety of factors such as:

Transfection efficiency

• Cell confluency
• Antibiotic selection conditions
• Quality and concentration of DNA
• Retargeting vector to integrase vector ratio

We strongly recommend including a retargeting reaction with the pJTI™ R4 Exp CMV EmGFP pA vector in your Jump-In™ experiment along with negative controls (no plasmid DNA, no integrase vector) so you can easily visualize the results and optimize the retargeting conditions.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Stable Cell Line Development, Targeted Integration
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): None
Vector Type: Jump-In Vectors
Form: Liquid
Product Size: 100 µg
Volume (Metric): 100 µl
Shipping Condition: Dry Ice
Constitutive or Inducible System: Constitutive

Contents & storage

Immediately upon receipt, store vector at -20°C

Documents

Manuals & protocols

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