LanthaScreen® Tb-anti-Histone H3K9me2 Antibody

Catalog number: A14151

Invitrogen™  Related applications: Target & Lead Identification & Validation

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Description

The LanthaScreen® Tb-anti-Histone H3K9me2 Antibody is a key component of the BacMam Histone H3K9me2 Cellullar Assay (A14161). When this terbium (Tb)-labeled antibody is paired with baculovirus-mediated (BacMam) expression of a Histone H3-GFP fusion protein, the TR-FRET readout enables sensitive detection of H3 lysine 9 dimethylation. The resulting high-throughput screening (HTS) compatible assay can be used to identify inhibitors of methyltransferases and demethylases acting on lysine 9 of histone H3 in the cell type of your choice.

LanthaScreen® Tb-anti-Histone H3K9me2 Antibody lets you:

• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Conserve precious cell samples with a homogenous assay format that can be easily miniaturized
• Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results
The LanthaScreen® Histone H3K9me2 antibody allows for the investigation of dimethylation at lysine 9 on histone H3. This enables screening for potential inhibitors of methyl transferases and demethylases associated with Lys9 dimethylation in their natural complexes in a physiologically relevant cell type.

BacMam and LanthaScreen® Convenience Saves Sample and Time
Assays are run in a fully homogenous, addition-only format without any washing, lysate transfer, or separation procedures required for traditional methods such as western blotting and ELISA, making it ideal for HTS applications. By using the same BacMam reagent for GFP-Histone H3, other histone H3 modifications (Ph, Me, Ac) can be monitored by simply exchanging the Terbium (Tb)-labeled antibody. In addition, the application of the LanthaScreen® technology includes all of the advantages of TR-FRET detection including reduced data noise, less interference from fluorescent compounds, and high sensitivity, which results in the need to use fewer cells than traditional western or ELISA methods.

For research use only. Not for human or animal therapeutic or diagnostic use.

Learn more about cellular epigenetics assays

Specifications

Form: Liquid
Label or Dye: Tb (Terbium)
Product Size: 1 mg
Concentration: 0.15 mg⁄ml, 1000 nM
Target Modification State: Methylated
Shipping Condition: Dry Ice

Contents & storage

Store the LanthaScreen® Tb-anti-histone H3K9me2 Antibody at -20°C

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Manuals & protocols

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