TargetSeq™ Oligo Blocker 1-16

Catalog number: A14177

Applied Biosystems™  Related applications: DNA Sequencing | SOLiD® Next-Generation Sequencing

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Description

TargetSeq™Oligo Blockers are designed to enable multiplexed library enrichment with all TargetSeq™Enrichment kits. The blocker oligonucleotides function to prevent cross-hybridization of the library adaptors during the solution-phase DNA probe capture step of the target enrichment protocol. TargetSeq™Oligo Blocker 1-16 contains an optimized mixture of P2 adaptors containing barcodes 1-16 and must be used in combination with TargetSeq™Oligo Blocker P1. These blocker oligos are sold individually but are used together during the library capture in order to block the adapters at both ends of the library molecules. TargetSeq™Oligo Blocker 1-16 is compatible with SOLiD® 4 and 5500 genetic analyzer fragment libraries that use the P1 sequencing adaptor and P2 adaptor barcodes 1-16.
• Optimized blocker design to reduce unwanted adaptor cross-hybridization during target enrichment for your 5500 Genetic Analyzer pipeline
• Conveniently provided at ready-to-use concentrations for fast reaction set-up and optimized performance
• Pre-mixed and validated to provide end users with significant time and cost savings

Optimized Blocker Designs
TargetSeq™Oligo Blocker 1-16 is a mixture of 16 blocker oligos that have been validated to provide optimal performance during multiplexed (4-plex) sample enrichment using full length barcoded libraries. Typical on-target read percentages are 70-85%, indicating that the blocker oligos are effectively preventing adaptor cross-hybridization during capture.

Convenience of Ready-to-Use Solution
TargetSeq™Oligo Blockers are optimized to provide exceptional and economical performance when combined with SOLiD® barcoded libraries (indexes 1-16) and SOLiD® 4 or 5500 Series genetic analysis systems. They diluted and ready to be added to capture reactions making experimental setup fast and easy. TargetSeq™enrichment systems are one of the first products lines to endorse multiplex sample capture (4-plex) using library barcoding and pre-capture sample pooling which dramatically lowers the per sample costs and simplifies the workflow.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Blocker Oligos
Key Function: Sequencing Target Enrichment (Block Cross-Hybridization)
Product Size: 24 reactions
For Use With (Equipment): SOLiD™ 4 System, 5500 Genetic Analyzer
Shipping Condition: Dry Ice

Contents & storage

Store at -15°C to -25°C

Documents

Manuals & protocols