pcDNA™3.4 TOPO® TA Cloning Kit

Catalog number: A14697

Invitrogen™  Related applications: Antibody & Cell Culture Production | Protein Expression | Transfection

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The pcDNA™ 3.4 TOPO® vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin®-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.

The pcDNA 3.4 TOPO® vector contains the following elements:

Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
TOPO® cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
Neomycin resistance gene for selection of stable cell lines with Geneticin®
pUC origin for high copy replication and maintenance of the plasmid in E. coli

The pcDNA3.4™ TOPO® TA Cloning Kit includes TOPO®-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot® TOP10 competent cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Protein Production, Constitutive Expression
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: pcDNA
Structure: Linear
Plasmid Type: High Copy Plasmid
Product Size: 1 kit
Special Elements: WPRE
Replication Origin: pUC Origin
Selection Marker Promoter: SV40 Promoter
Selection Marker (Eukaryotic): NeoR
Constitutive or Inducible System: Constitutive
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)

Contents & storage

Store pcDNA™3.4-TOPO® vector and all cloning reagents at -20°C.
Store One Shot® TOP10 Chemically Competent E. coli cells at -80°C.

Documents

Manuals & protocols

Recommended products