RiboMinus™ Eukaryote System v2

Catalog number: A15026

Ambion™  Related applications: Epigenetic Sequencing | RNA Extraction | RNA Sequencing

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Description

The RiboMinus™ Eukaryote System v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the cytoplasmic (5S, 5.8S, 18S, and 28S) and mitochondrial (12S and 16S) ribosomal RNA from total RNA. This selection system contrasts with poly-A and cap binding methods, which introduce bias and select for only part of the transcriptome.

Features of the RiboMinus™ Eukaryote System v2 include:

• Magnetic bead-based purification for rapid, seamless purification
• A 1-hour workflow
• An input range of 1–5 µg

The RiboMinus™ Eukaryote System v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the RiboMinus™ Eukaryote System v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The RiboMinus Eukaryote System v2 is designed for the removal of human, mouse, and rat rRNA. A magnetic bead-based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non-coding RNA. For column-based purification, please see the RiboMinus™ Eukaryote Kit v2. The RiboMinus Eukaryote System v2 has an RNA input range of 1–5 µg. For lower inputs (down to 100 ng), the Low Input RiboMinus™ Eukaryote System v2 is recommended.

The RiboMinus™ Eukaryote System v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Mouse, Human, Rat
Product Size: 12 reactions
Final Product: Transcriptome RNA
Workflow Step: Sample Enrichment
Sequencing Type: Transcriptome Sequencing
For Use With (Equipment): Illumina® Genome Analyzer II (GA II), Ion PGM™ System, Ion Proton™ System
Starting Material (Amount): 1 - 5 µg total RNA

Contents & storage

• RiboMinus™ Eukaryote Probe Mix v2 (store at -20°C)
• RiboMinus™ Magnetic Beads (store at +4°C)
• 2X Hybridization Buffer (store at room temperature)
• Binding Solution Concentrate (store at room temperature)
• Wash Solution Concentrate (store at room temperature)
• Nucleic Acid Binding Beads (store at +4°C)

Documents

Manuals & protocols

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