TaqPath™ qPCR Master Mix, CG

Catalog number: A15297

Applied Biosystems™  Related applications: Diagnostic Development | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling | miRNA & Non-Coding RNA Analysis

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TaqPath™ qPCR Master Mix, CG, is designed and all lots are functionally tested to help ensure lot-to-lot reproducibility for Ct consistency and dynamic range across a wide variety of assays. This General Purpose Reagent is manufactured according to applicable CFR requirements and labeled "For Laboratory Use". With stringent quality and premier performance, TaqPath™ qPCR Master Mix is a superior choice for your diagnostic testing or development needs.

Features of the TaqPath™ qPCR Master Mix include:

• Efficient and linear detection of up to 8 logs with gene expression or miRNA assays*
• Enables reliable detection of low-copy templates with reproducible CT results
• Robust multiplexing performance with exogenous or endogenous targets
• Manufactured with stringent production and process controls to help ensure lot-to-lot consistency
• Labeled "For Laboratory Use"

TaqPath™ qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N glycosylase (UNG), dNTPs with dUTP, ROX™ dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Validated with a Breadth of Workflows
TaqPath™ qPCR Master Mix, CG, has been validated to provide high specificity and dynamic range for use in multiple real-time PCR applications. The formulation can be run in either fast or standard cycling conditions on a wide variety of qPCR platforms. Figure 1 demonstrates the excellent PCR linearity over an 8-log input range of template with both gene expression (Figure 1a) and miRNA assays(Figure 1b).

In addition, TaqPath™ qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate (Figure 2). TaqPath™ qPCR Master Mix has been benchmarked against similar competitor master mixes and demonstrates equivalent or better sensitivity and dynamic range across a variety of targets (Figure 3).

Reproducible Sensitive Detection
We understand the importance of reliably detecting low-copy targets for your test quality and data interpretation. TaqPath™ qPCR Master Mix, CG, helps generate significant and reproducible CT values for ≤10 copy detection. Figure 4 shows the consistent CT results obtained from three unique lots when detecting 10-copy inputs. This lot-to-lot CT consistency is preserved across multiple assays with different attributes and expression levels to maximize confidence in your results (Figure 5).

Optimized for Multiplexing
Simultaneous amplification of multiple assays can be beneficial not only as a control to normalize and detect anomalies in experiments, but also to improve efficiency and cost-savings for labs. TaqPath™ qPCR Master Mix has been optimized with enzymes and component concentrations to facilitate multiplexing while preserving specificity, and it is validated for duplex performance in each manufacturing lot (Figure 6).

Inhibitor Tolerant
Unlike other master mixes on the market, TaqPath™ qPCR Master Mix’s unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath™ master mixes demonstrate increased tolerance to inhibitors compared to competitor mixes (Figure 7).

Manufacturing Production and Process Controls
TaqPath™ qPCR Master Mix is manufactured under a quality management system that utilizes traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath™ product manufacturing is designed to produce consistent product performance lot after lot.

General Purpose Reagent
For customers in the USA, TaqPath™ 1-Step RT-qPCR Master Mix is a General Purpose Reagent and "For Laboratory Use".

*Dynamic range is a property of both the assay and template concentration in the sample, as well as in the master mix, thus individual results may vary.
For Laboratory Use


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, QuantStudio™ Dx, StepOne™, StepOnePlus™, ViiA™ 7 System
Reaction Speed: Fast, Standard
PCR Method: qPCR
Sample Type (General): DNA, cDNA
Form: Liquid
Format: Bottle(s)
Hot Start: Built-In Hot Start
Number of Reactions: 500 reactions
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: Fast DNA Polymerase
Primer-Probe Compatibility: TaqMan®
Product Line: TaqPath™
Product Size: 1 x 5 mL

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols