CytoTune™ EmGFP Sendai Fluorescence Reporter

Catalog number: A16519

Invitrogen™  Related applications: Stem Cell Engineering & Reprogramming

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Description

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits. This vector expresses the next-generation Aequorea victoria EGFP gene, encoding the emerald green fluorescent protein (EmGFP). The cell will express the EmGFP, which is bright, photostable, and ideal for testing transduction.

The CytoTune™ EmGFP Sendai Fluorescence Reporter enables a better understanding of how both the original CytoTune™-iPS Sendai Reprogramming kits and the newer CytoTune™-iPS 2.0 Sendai Reprogramming Kit transduces various cell types. The Sendai particle enters a cell via its HN envelope protein that recognizes sialic acid and is widely expressed in mammalian cells, allowing Sendai virus to target a wide range of cell types. The Sendai virus vectors do not enter the nucleus, thus avoiding any DNA phase, as the viral genome remains as RNA in the cytoplasm.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: Proprietary
Reporter: GFP (EmGFP)
Product Size: 1 pack
Delivery Method: Sendai virus
Sub-Cellular Localization: Cytoplasm

Contents & storage

1 tube, store at -68 to -85°C.

Documents

Manuals & protocols