Human Episomal iPSC Line

Catalog number: A18945

Gibco™  Related applications: Stem Cell Culture

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Description

The Gibco® Human Episomal iPSC Line was derived from CD34+ cord blood using a three-plasmid, seven-factor (SOKMNLT; SOX2, OCT4 (POU5F1), KLF4, MYC, NANOG, LIN28, and SV40L T antigen) EBNA-based episomal system. This iPSC line is considered to be zero foot-print as there was no integration into the genome from the reprogramming event.It has been shown to be free of all reprogramming genes and will provide researchers with a control line to compare and study a multitude of aspects of stem cell research.

The Gibco® Human Episomal iPSC Line has a normal karyotype and endogenous expression of pluripotent markers like Oct4, Sox2, and Nanog (as shown by RT-PCR) and Oct4, SSEA4, TRA-1-60 and TRA-1-81 (as shown by ICC). Whole genome expression and epigenetic profiling analyses demonstrated that this episomal hiPSC line is molecularly indistinguishable from human embryonic stem cell lines (Burridge et al., 2011). In directed differentiation and teratoma analyses, these hiPSCs retained their differentiation potential for the ectodermal, endodermal, and mesodermal lineages (Burridge et al., 2011). In addition, vascular, hematopoietic, neural, and cardiac lineages were derived with robust efficiencies (Burridge et al., 2011).

Reference
Burridge, P.W., Thompson, S., Millrod, M.A., et al. 2011. A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One, April 2011, Volume 6, Issue 4, e18293.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Human
Quantity: 1 vial(s)
Cell Type: Stem Cells (iPS - Induced Pluripotent Stem)
Culture Type: Feeder Cell-Free
Product Size: 1 x 106 cells⁄vial
Classification: Animal Origin-Free
Number of Cells: 1 x 106
Media Recommendation: Essential 8

Contents & storage

1 vial cells, store in liquid nitrogen (vapor phase).

Documents

Manuals & protocols