Foxp3 Transcription Factor Staining Buffer Kit

Catalog number: A25866A

Molecular Probes™  Related applications: Flow Cytometry

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Description

The Foxp3 Transcription Factor Buffer Kit has been specially formulated to work with the APC (A18629), FITC (A18662), and PE (A18690) conjugates of Foxp3 Mouse Anti-Mouse Monoclonal Antibody (clone 3G3) for optimal observation of intranuclear Foxp3 in flow cytometry applications.

The kit contains 3 buffers:
1) Foxp3 Fixation Transcription Factor and Permeabilization Buffer A (A24217, a 4X concentrate)
2) Foxp3 Transcription Factor Fixation and Permeabilization Buffer B (A24218, a diluent)
3) Foxp3 Transcription Factor Wash Buffer (A24261, 10X)

How It Is Used
The Foxp3 Transcription Factor Fixation and Permeabilization Buffer A is supplied as a 4X stock solution and must be diluted with Foxp3 Transcription Factor Fixation and Permeabilization Buffer B prior to use.

The Foxp3 Transcription Factor Wash Buffer is supplied as a 10X stock solution. The 10X stock solution should be diluted to a 1X working concentration in PBS containing 1% BSA and used for wash and intranuclear Foxp3 staining steps following fixation and permeabilization with 1X Foxp3 Fixation and Permeabilization buffers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 1 kit
Reagent Type: Permeabilization Reagent, Fixation Reagent

Contents & storage

• 50 mL Buffer A
• 160 mL Buffer B
• 100 mL Wash Buffer

Store in refrigerator (2–8°C).

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Manuals & protocols

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