Ion Torrent™

Oncomine™ Comprehensive Library Assay

Catalog number:  A29226

 Related applications: Pharma & Biopharma | Real Time PCR (qPCR)

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The Oncomine™ Comprehensive Library Assay is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 143 unique genes to help inform drug discovery research, and clinical trial research programs. It provides the reagents for library construction and three pools—two DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per pool, the assay covers more than 2,500 amplicons and 170 biomarkers.

The Oncomine Comprehensive Library Assay includes oligos for library construction. For a kit that also includes reagents and consumables for next-generation sequencing, please see the Oncomine Comprehensive Assay.

• Enables analysis of variants across 143 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool, isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and >300 FFPE samples
• NGS results concordant with verified single gene tests [1]
• Additional relevant variants detected
• Content driven by the Oncomine™ Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published clinical trials

From sample to answer
The Oncomine Comprehensive Library Assay is part of an NGS workflow that helps researchers filter variant results from hundreds, to just a few key cancer driver variants. Enabled by Oncomine™ informatics, this filtering, and as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Comprehensive Assays >

1. Hovelson et al., 2015. Development and validation of a scalable next-generation sequencing system for assessing relevant somatic variants in solid tumors. Neoplasia 17:385-399.

Package components

Unit size


A26120 Catalog number:A26120 1
A26145 Catalog number:A26145 1
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion Chef™ System, Ion S5™ System
Library Type: Targeted Sequencing Library
Starting Material: PCR Products
Product Line: Oncomine™
Product Size: 48 samples
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

• Ion AmpliSeq Library Kit 2.0, multiple storage temps
• Oncomine Comprehensive Panel, room temperature
• Oncomine Comprehensive DNA Panel, room temperature
• Oncomine Comprehensive RNA Panel, room temperature


Manuals & protocols