Oncomine™ Focus Assay, Select Library

Catalog number:  A29228

Ion Torrent™  Related applications: Cancer Genomics | Ion Torrent™ Next-Generation Sequencing

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The Oncomine Focus Assay, Select Library, is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables the detection of variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. The assay provides reagents for library construction and two pools—one DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single sequencing run.

The Oncomine Focus Assay, Select Library, includes panel primers and reagents for library construction and normalization. The Select Library reagents, manufactured to rigorous standards in our ISO 13485-compliant facility, are sufficient for 48 samples, including 16 barcodes, facilitating the combination of up to 8 samples of DNA and RNA in a single run. The libraries are compatible with automated template preparation and chip loading on the Ion Chef System. For a kit that also includes reagents and consumables for next-generation sequencing on the Ion PGM System using the Ion OneTouch 2 System, please see the Oncomine Focus Assay, 318 Solution.

From sample to answer
The Oncomine Focus Assay, Select Library, is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Focus Assays and Oncomine Oncology.

For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion Chef™ System, Ion OneTouch™ 2 System, Ion PGM™ System, Ion S5™ System
Library Type: Targeted Sequencing Library
Workflow Step: Library Generation
Starting Material: DNA, RNA
Product Line: Oncomine™
Product Size: 48 samples
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

• 5X Oncomine Focus DNA Assay, 48 reactions, store at -5 to -30°C
• 5X Oncomine Focus RNA Assay, 48 reactions, store at -5 to -30°C
• Ion PGM Select Library equalizer, store at 2–8°C
• Ion PGM Select Library reagents, store at -5 to -30°C

NOTE: SuperScript for cDNA synthesis not included. It must be purchased separately (Cat Nos. A26123 or 11754050).


Manuals & protocols

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