Oncomine™ Focus Library Assay

Catalog number: A29228

Ion Torrent™  Related applications: Real Time PCR (qPCR)

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Description

The Oncomine™ Focus Library Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables you to detect variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. It provides reagents for library construction and three pools—two DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single workflow.

The Oncomine Focus Library Assay includes oligos for library construction. For a kit that also includes reagents and consumables for next-generation sequencing, please see the Oncomine Focus Assay.

• Simultaneous detection of hundreds of variants across 52 genes relevant to solid tumors
• Detect hotspots, SNVs, indels, CNVs, and gene fusions
• Robust performance from as little as 10 ng isolated from FFPE samples, including fine needle biopsies
• Enables results from up to 6 samples per chip for DNA and RNA in a single workflow
• Content driven by the Oncomine™ Knowledgebase and experienced scientists helps assure coverage of key targets for cancer clinical research
• Includes Ion Select™ consumables and reagents manufactured to rigorous standards in our ISO 13485-compliant facility

From sample to answer
The Oncomine Focus Library Assay is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine™ informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Focus Assays >

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion Chef™ System, Ion OneTouch™ 2 System, Ion PGM™ System, Ion S5™ System
Library Type: Targeted Sequencing Library
Workflow Step: Solid Tumor DNA &  RNA Synthesis Library Generation
Starting Material: DNA I RNA
Sequencing Type: Genome & DNA & RNA Sequencing
Product Line: Oncomine™
Product Size: 48 samples
Shipping Condition: Dry Ice

Contents & storage

• Ion PGM Select Lib Reagents, -5 to -30°C
• Ion PGM Select Lib Equalizer, 2–8°C
• Oncomine Focus DNA Assay, -5 to -30°C
• Oncomine Focus RNA Assay, -5 to -30°C

Documents

Manuals & protocols

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