MAXIscript® SP6/T7 Transcription Kit

Catalog number: AM1320

Invitrogen™  Related applications: In Vitro Transcription | Nucleic Acid Gel Electrophoresis & Blotting

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Description

The Ambion® MAXIscript® SP6/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 15 reactions of each enzyme.

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript® In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Starting Material: Linearized DNA, PCR Products
Promoter: T7, SP6
RNA Yield: 2-6 µg
Final Product: Probe, In Vitro Transcribed RNA
Product Size: 30 reactions
Shipping Condition: Dry Ice

Contents & storage

SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Documents

Manuals & protocols