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      Invitrogen™

      MEGAscript™ SP6 Transcription Kit

      Catalog number:  AM1330

       Related applications: In Vitro Transcription

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      Description

      Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 40 reactions.

      • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
      • Amplifies aRNA for gene array analysis and other applications
      • Efficiently incorporates many modified nucleotides

      A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase.

      Accessory Products:
      MessageAmp™ aRNA Amplification Kits are powered by MEGAscript® technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, Ambion recommends using the MEGAclear™ Kit (SKU#AM1908) and the MEGAclear™-96 Kit (SKU#AM1909).
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Starting Material: Linearized DNA, PCR Products
      Promoter: SP6
      RNA Yield: 100 µg
      Final Product: In Vitro Transcribed RNA
      Product Line: Ambion™, MEGAscript®
      Product Size: 40 reactions
      Shipping Condition: Dry Ice

      Contents & storage

      SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

      Documents

      Manuals & protocols