QuantumRNA™ Classic II 18S Internal Standard with Manual

Catalog number: AM1717M

Ambion™

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Description

The Ambion® QuantumRNA™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Classic II 18S Internal Standards produce a 324 bp PCR product.

Accessory Products:
The QuantumRNA™ Classic 18S Internal Standard (SKU# AM1716) produce a 489 bp PCR product, and the QuantumRNA™ Universal 18S Internal Standard (SKU# AM1718) function across the broadest range of eukaryotes, including animals, plants, and many protozoa and produce a 315 bp PCR product.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Tube(s)
Species: Mouse
Includes: Control Primers & Template(s)
Gene Symbol: 18S
Target Gene: 18S rRNA
Label or Dye: Unconjugated
Product Size: 100 reactions
Control Template: Total RNA from Mouse Liver

Contents & storage

18S Primer Pair and 18S Competimer™s are stored at –20°C. Control Template RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.

Documents

Manuals & protocols