RiboPure™ RNA Purification Kit

Catalog number: AM1924

Ambion™  Related applications: RNA Extraction

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Description

The Ambion® RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells. The kit combines TRI Reagent® with glass fiber filter purification to yield exceptionally pure RNA, free of residual proteins and lipids. The kit includes sufficient reagents for 50 purifications from 1–100 mg of tissue or 0.1–20 x 106 cultured cells each.

• Yields the highest purity RNA attainable
• Ideal for the most stringent downstream applications
• Handles problematic samples with ease
• Fast (less than 30 minutes) and easy procedure

RNA isolated using the RiboPure™ Kit is suitable for use in all common downstream applications, including cDNA synthesis, real-time and end-point RT-PCR, microarray analysis, northern blots, and RNase protection assays. The RiboPure™ protocol is especially suited for processing samples that are difficult to disrupt, or samples that have large amounts of ribonuclease or other contaminants. Samples are first homogenized in TRI Reagent®, a monophasic solution containing phenol and guanidine thiocyanate, to rapidly lyse cells and inactivate nucleases. Addition of bromochloropropane (BCP) results in separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, while DNA and protein remain in the interphase and organic phase. The aqueous phase is removed and passed through a glass fiber filter that binds the RNA. The filter is washed, and the RNA is eluted. The procedure is compatible with tissues that have been stored in Ambion's RNAlater®. Total RNA yield is typically 100–500 µg per 100 mg of tissue, depending on the type of tissue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Cells, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), In Vitro Translation, Microarray Analysis, Northern Blotting, cDNA Library Construction, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR)
Product Size: 50 preps
Final Product: Total RNA
Yield (up to): 500 µg
Isolation Technology: Spin Column, Organic Extraction
Sample Type (Specific): Mammalian Cells, Tissue (Mammalian)
Starting Material (Amount): Up to 100 mg tissue, Up to 2 x 10^7 cells
Shipping Condition: Room Temperature

Contents & storage

Store TRI Reagent at 4°C. Store Filter Cartridges, Collection Tubes (2 mL), Wash Solution Concentrate and Elution Buffer at room temperature.

Documents

Manuals & protocols

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