M-MLV Reverse Transcriptase (100 U/µL)

Catalog number: AM2044

Invitrogen™  Related applications: Reverse Transcription

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Description

M-MLV Reverse Transcriptase (RT) is commonly used in the first step of RT-PCR as well as for primer extension experiments. In the presence of a single-stranded RNA or DNA template, primer, and deoxyribonucleotides, this enzyme will extend the oligonucleotide primer, producing a complementary strand.

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is derived from a cloned region of the pol gene of M-MLV and isolated from an E. coli strain overexpressing this construct.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Optimal Reaction Temperature: 37° C
Final Product Size(s): 7 kb or less
Reverse Transcriptase: M-MLV Reverse Transcriptase
Ribonuclease H Activity: None
Enzyme Function: RNA dependent DNA Polymerase
Product Line: Ambion™
Product Size: 10,000 units
Shipping Condition: Dry Ice
Template: RNA-DNA Hybrid, ssDNA, ssRNA

Contents & storage

Contains:
• M-MLV Reverse Transcriptase (10,000 units)
• 500 µL 10X First Strand Synthesis Buffer

Store at -20°C. Do not store in a frost-free freezer.

Documents

Manuals & protocols

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