This product will be discontinued on September 1, 2016. For superior performance, we recommend you switch to SUPERSCRIPT IV 10,000 units.

ArrayScript™ Reverse Transcriptase

Catalog number: AM2049

Invitrogen™  Related applications: Reverse Transcription

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Description

Ambion® ArrayScript™ Reverse Transcriptase is an M-MLV reverse transcriptase that has been engineered to produce high yields of full-length cDNA. 10,000 U are supplied along with a 10X Reaction Buffer. Reverse transcriptases (RTs) are used in a variety of molecular biology experiments where success can depend upon full-length cDNA synthesized during the reverse transcription reaction. In RNA amplification experiments, up to twice as much cRNA is synthesized when the first-strand cDNA is generated with ArrayScript™ RT compared to wild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases when low amounts of total RNA (~100 ng) are used (see figure). This can eliminate the need to perform two rounds of cRNA amplification when working with limiting amounts of total RNA. ArrayScript™ is also suitable for other RT and RT-PCR applications where high yields of full-length cDNA are required, such as cDNA library construction, 5' RLM-RACE, etc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Optimal Reaction Temperature: 37° C
Final Product Size(s): 7 kb or less
Reverse Transcriptase: ArrayScript™ Reverse Transcriptase
Ribonuclease H Activity: None
Template: RNA-DNA Hybrid, ssDNA, ssRNA
Product Size: 10,000 units
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Dry Ice

Contents & storage

Store at -20°C.

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