TURBO™ DNase (2 U/µL)

Catalog number: AM2239

Ambion™  Related applications: Real Time PCR (qPCR) | Reverse Transcription

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Description

TURBO™ DNase cleaves double-stranded DNA nonspecifically to leave 5' phosphorylated oligodeoxynucleotides. It has increased affinity for DNA-binding and remains active in the presence of salt. Features of TURBO™ DNase include:

• Up to 50x more activity and 350% greater catalytic efficiency
• Efficiently degrades DNA in solutions containing up to 0.25 M salt
• Efficiently digests DNA to oligonucleotides
• Vastly superior in clearing DNA templates from in vitro transcription reactions
• RNase-free and recombinant in origin

Using TURBO™ DNase
DNase I is commonly used to clear DNA contamination from RNA samples prior to RT-PCR. Conventional DNase I has a poor affinity for DNA and cleaves DNA of low concentration very inefficiently. In addition, DNase I is very salt-sensitive; as little as 20 mM NaCl can reduce the activity of the enzyme by 30%. Finally, DNase I is purified from bovine pancreas, one of the richest natural sources of RNase A. The threat of contaminating RNase activity in DNase I preparations requires that the enzyme be exhaustively purified. In spite of these limitations, the DNase I that researchers use today is the very same enzyme that was first characterized by Kunitz more than a half-century ago.

A different DNase with superior properties to wild-type DNase I
TURBO™ DNase was developed using a protein engineering approach that introduced amino acid changes into the DNA binding pocket of wild-type DNase I. These changes markedly increase the affinity of the protein for DNA. The result is a versatile enzyme that has a 6-fold lower Km for DNA, and an ability to maintain at least 50% of peak activity in solutions approaching 200 mM monovalent salt, even when the DNA concentration is in the nanomolar (nM) range. When in vitro transcription reactions are treated with either DNase I or TURBO™ DNase, TURBO™ DNase removes 63x more of the input plasmid DNA template than the wild-type enzyme. The proficiency of TURBO™ DNase in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination. This becomes important for complete removal of DNA from a sample, since the cleavable DNA substrate is reduced as the DNase reaction proceeds. TURBO™ DNase thus has a functional advantage over wild-type DNase due to its superior affinity for DNA. This is best exploited in RT-PCR applications, where even a few copies of DNA can lead to a false positive outcome by PCR.

Accessory product
TURBO DNA-free™ (Cat. No. AM1907) is available. It contains TURBO™ DNase along with reagents to inactivate the enzyme and remove divalent cations from the sample post-digestion.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: DNase
Product Size: 5,000 units
Shipping Condition: Dry Ice

Contents & storage

TURBO™ DNase and 10X Reaction Buffer should be stored at –20°C.

Documents

Manuals & protocols

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