This product will be discontinued on September 1, 2016. We recommend you switch to RNase A, DNase and protease-free.

RNase A (1 μg/mL)

Catalog number: AM2274

Invitrogen™  Related applications: DNA Extraction

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion® RNase A, RNA-Grade cleaves 3' of single-stranded C and U residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 µL (1 µg/mL). In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding protein.

Unit Definitions:
One unit is defined as the amount of RNase A required to yield an increase in absorbance at 286 nm of 0.0146 absorbance units per min in a 1 mL volume. Assay Conditions: 100 mM Tris-acetate (pH 6.5), 1 mM EDTA, and 1 mM cyclic 2', 3'-CMP.

Accessory Products:
Other RNA-Grade ribonuclease available include RNase V1 (SKU# AM2275) ,which cleaves base-paired nucleotides and RNase T1 (SKU# AM2283), which cleaves 3' of single-stranded G residues. Combinations of the single- and double-stranded specific ribonucleases can provide rapid analysis of the physical structure of an interesting RNA.
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: RNase
Product Size: 200 µL
Shipping Condition: Dry Ice

Contents & storage

RNase A, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation⁄Inactivation Buffer, and Gel Loading Buffer II should all be stored at


Manuals & protocols