RNase I (cloned) 100 U/μL

Catalog number: AM2294

Invitrogen™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting

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Description

Ambion® RNase I efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues. RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. Supplied in one tube of 10,000 U (100 U/ µL). RNase I will degrade any RNA to a mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays. This RNase I is rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity.

Unit Definition:
One unit is the amount of enzyme required to produce 1 µg of acid-soluble material from mouse liver RNA in 30 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: RNase
Product Size: 10,000 units
Shipping Condition: Dry Ice

Contents & storage

Store at –20°C

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