SUPERase• In™ RNase Inhibitor (20 U/μL)

Catalog number: AM2696

Ambion™  Related applications: In Vitro Transcription | Real Time PCR (qPCR) | Reverse Transcription

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Description

SUPERase• In™ RNase Inhibitor is a protein-based inhibitor of nonhuman origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1. Features of SUPERase• In™ RNase Inhibitor:

• Completely removes RNase contamination from glass and plastic surfaces
• Excels at removing high levels of RNase contamination where similar products fail
• Proven effective at removing high concentrations of dried-on RNase A
• Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free

SUPERase• In™ RNase Inhibitor can be used in any application where RNase contamination could be problematic. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERase• In™ is the most effective RNase inhibitor available, providing a higher level of protection against degradation.

SUPERase• In™ does not interfere with other enzymes such as RNA polymerases, reverse transcriptase, or Taq DNA polymerase. Additionally, SUPERase• In™ is active up to 65°C and over a pH range of 5.5 to 8.5.

If you are plannning to use this product with standard antibody purification methods, please contact our technical support to discuss experimental design.

Unit definition
SUPERase• In™ RNase Inhibitor at 1 U/µL will block the degradation of 0.1 µg/µL labeled RNA by 2.5 pg/µL of RNase A, 2.5 pg/µL of RNase I, and 0.0075 U/µL of RNase T1, for 4 hours at 37°C, in 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM EDTA. Analysis is by denaturing PAGE. SUPERase
• In is currently the only ribonuclease inhibitor for which the unit activity is defined by such a functional assay.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 10,000 units
Shipping Condition: Dry Ice

Contents & storage

Supplied in a single tube that should be stored at -20°C.

Storage buffer: 2 mM KH2PO4, 8 mM Na2HPO4, 2.7 mM KCl, 137 mM NaCl, pH 7.4 in 50% glycerol

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